Fibrinolytic Activity in Mongrel Dogs
نویسندگان
چکیده
Background. Numerous investigators have observed that pulmonary emboli are rapidly lysed in a canine model system. This study was undertaken to delineate the unique mechanism that accounts for the rapid dissolution of pulmonary emboli in mongrel dogs. Methods and Results. Canine plasminogen activator (PA) activity (2.6± 1.1 IU/mL acidified platelet-poor plasma [PPP], <0.3 IU/mL acidified whole blood serum [WBSJ, mean-SD; n=6) and PA inhibitor activity (6.1±2.6 U/mL PPP, 35.4±7.8 U/mL WBS; n=6) were determined in standard plasminogen-based chromogenic assays. Analysis of canine PPP, WBS, platelet lysates, and primary canine endothelial cell (EC) cultures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fibrin autography revealed a plasminogen-dependent lytic zone at 45-kd relative molecular mass that was shown to be related to urokinase-type PA (u-PA) by its selective inhibition through amiloride. Analysis of canine platelets on standard '5I fibrin plate assays revealed a net fibrinolytic activity. In a clot lysis assay system, canine platelets were able to stimulate fibrinolysis when layered on the outside of fibrin clots containing autologous PPP. Moreover, net fibrinolytic activity of primary canine pulmonary artery endothelial cells
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